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live dead tm fixable aqua dead cell stain kit  (Thermo Fisher)


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    Thermo Fisher live dead tm fixable aqua dead cell stain kit
    Live Dead Tm Fixable Aqua Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aqua+live/Bovine+Serum+Albumin/bio_rxiv__64898__2026__06__05__730120-39-4-12
    Average 99 stars, based on 1 article reviews
    live dead tm fixable aqua dead cell stain kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Sex‐specific characterization of aortic function and inflammation in a new diet‐induced mouse model of metabolic syndrome
    Article Snippet: Samples were then passed through a sterile 70 μm filter (BD Biosciences, USA) and cells were pelleted by centrifugation at 1500 × g RPM for 5 min at 4°C. .. Aortic cell suspensions were stained with an Aqua Live/dead cell viability stain (Invitrogen, USA; 1 in 1000 dilution in PBS) for 15 min at room temperature, followed by a master mix containing a cocktail of antibodies in MACS buffer (0.5% bovine serum albumin and 2 mM EDTA in PBS; full list of antibodies in Table ). ..

    Article Title: Optimization of mouse kidney digestion protocols for single-cell applications
    Article Snippet: .. All single-cell suspensions were loaded onto 96-well U-bottomed plates and stained with aqua live/dead viability stain (1:1,000 dilution in PBS; Thermo Fisher, Victoria, Australia) at room temperature for 15 min. ..

    Article Title: Optimization of mouse kidney digestion protocols for single-cell applications.
    Article Snippet: Single-cell technologies such as flow cytometry and single-cell RNA sequencing have allowed for comprehensive characterization of the kidney cellulome.. However, there is a disparity in the various protocols for preparing kidney single-cell suspensions.. We aimed to address this limitation by characterizing kidney cellular heterogeneity using three previously published single-cell preparation protocols.

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: For intracellular staining, cells were treated with GolgiStop (BD Biosciences) for 5 h prior to staining. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 BV605 RM4-5, CD8 FITC 53-6.7, CD44 APC IM7, CD69 PE-Cy7 H1.2F3, CD134 BV786 OX-86, CD137 PE 1AH2, and CD45R/B220 Per-CP 5.5 RA3-6B2 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained with aTNFα BV650 MP6-XT22 (BD Bioscience).

    Article Title: Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primates
    Article Snippet: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207) to yield RBD-Tetramers. .. Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen, L34957), anti-human IgM (FITC, clone: G20–127, BD cat# 555782, 1:5), anti-human IgG (PE-Cy7, clone: G18–145, BD cat# 561298, 1:20), anti-human CD3 (AF700, clone: SP34-2, BD cat# 557917, 1:20), anti-human PD-1 (BV650, clone: EH12.1, BD cat# 564104, 1:20), anti-human CD20 (PE/Dazzle 594, clone: 2H7, BioLegend cat# 302348, 1:20), anti-human CD4 (APC-Cy7, clone: OKT4, BioLegend cat# 317418, 1:20) and anti-human CXCR5 (PerCP-eF710, clone: MU5UBEE, ThermoFisher cat# 46-9185-42, 1:20). .. Cells were fixed/permeabilized using Transcription Factor Staining Buffer Set (ThermoFisher, cat# 00-5521-00) and further stained with anti-human Bcl-6 (PE, clone: 7D1, BioLegend cat# 358504, 1:20) and anti-human Ki-67 (BV421, clone: 11F6, BioLegend cat# 151208, 1:20).

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: GolgiStop (BD Biosciences) was added for the last 10 h of the assay. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 Ax700 RM4-5, CD8 APC-H7 53-6.7, and CD44 APC IM7 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained for intracellular cytokines with IFNγ PE-Cy7 XMG1.2, TNFα BV650 MP6-XT22, and IL-4 BV786 11B11 (BD Bioscience).

    Article Title: Virion proteomics of genetically intact HCMV reveals a regulator of envelope glycoprotein composition that protects against humoral immunity
    Article Snippet: Mouse anti-His (clone His.H8) Thermo Fisher MA1-21315 MACS anti-IgG MicroBeads Miltenyi Biotec 130-048-401 MACS LS Columns Miltenyi Biotec 130-042-401 MACS Separation Buffer Miltenyi Biotec 130-091-221 MACS Pre-Separation Filters (70μM) Miltenyi Biotec 130-095-823 .. Aqua live/dead Thermo Fisher L34966 GolgiStop BD Biosciences 554724 PNGase F NEB P0704 Endo H NEB P0702 Agarose anti-V5 tag Abcam ab1229 Agarose anti-HA tag Abcam ab214758 FastGene 2x IC Green qPCR Universal Mix Geneflow P8-0058 CellBrite® Steady 550 Membrane Staining Kit biotium 30107 Fig. S1. ..

    Article Title: Sex-specific effects of a high fat diet on aortic inflammation and dysfunction
    Article Snippet: Samples were then passed through a sterile 70 μm filter (BD Biosciences, USA) and cells were pelleted by centrifugation at 1500 RPM for 5 min at 4 °C. .. Aortic cell suspensions were stained with an Aqua Live/dead cell viability stain (Invitrogen, USA; 1 in 1000 dilution in PBS) for 15 min at room temperature, followed by a master mix containing a cocktail of antibodies in MACS buffer (0.5% bovine serum albumin and 2 mM EDTA in PBS; full list of antibodies in Suppl Table ). ..

    Magnetic Cell Separation:

    Article Title: Sex‐specific characterization of aortic function and inflammation in a new diet‐induced mouse model of metabolic syndrome
    Article Snippet: Samples were then passed through a sterile 70 μm filter (BD Biosciences, USA) and cells were pelleted by centrifugation at 1500 × g RPM for 5 min at 4°C. .. Aortic cell suspensions were stained with an Aqua Live/dead cell viability stain (Invitrogen, USA; 1 in 1000 dilution in PBS) for 15 min at room temperature, followed by a master mix containing a cocktail of antibodies in MACS buffer (0.5% bovine serum albumin and 2 mM EDTA in PBS; full list of antibodies in Table ). ..

    Article Title: Sex-specific effects of a high fat diet on aortic inflammation and dysfunction
    Article Snippet: Samples were then passed through a sterile 70 μm filter (BD Biosciences, USA) and cells were pelleted by centrifugation at 1500 RPM for 5 min at 4 °C. .. Aortic cell suspensions were stained with an Aqua Live/dead cell viability stain (Invitrogen, USA; 1 in 1000 dilution in PBS) for 15 min at room temperature, followed by a master mix containing a cocktail of antibodies in MACS buffer (0.5% bovine serum albumin and 2 mM EDTA in PBS; full list of antibodies in Suppl Table ). ..

    Single Cell:

    Article Title: Optimization of mouse kidney digestion protocols for single-cell applications
    Article Snippet: .. All single-cell suspensions were loaded onto 96-well U-bottomed plates and stained with aqua live/dead viability stain (1:1,000 dilution in PBS; Thermo Fisher, Victoria, Australia) at room temperature for 15 min. ..

    Article Title: Optimization of mouse kidney digestion protocols for single-cell applications.
    Article Snippet: Single-cell technologies such as flow cytometry and single-cell RNA sequencing have allowed for comprehensive characterization of the kidney cellulome.. However, there is a disparity in the various protocols for preparing kidney single-cell suspensions.. We aimed to address this limitation by characterizing kidney cellular heterogeneity using three previously published single-cell preparation protocols.

    Bioprocessing:

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: For intracellular staining, cells were treated with GolgiStop (BD Biosciences) for 5 h prior to staining. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 BV605 RM4-5, CD8 FITC 53-6.7, CD44 APC IM7, CD69 PE-Cy7 H1.2F3, CD134 BV786 OX-86, CD137 PE 1AH2, and CD45R/B220 Per-CP 5.5 RA3-6B2 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained with aTNFα BV650 MP6-XT22 (BD Bioscience).

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: GolgiStop (BD Biosciences) was added for the last 10 h of the assay. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 Ax700 RM4-5, CD8 APC-H7 53-6.7, and CD44 APC IM7 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained for intracellular cytokines with IFNγ PE-Cy7 XMG1.2, TNFα BV650 MP6-XT22, and IL-4 BV786 11B11 (BD Bioscience).

    Blocking Assay:

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: For intracellular staining, cells were treated with GolgiStop (BD Biosciences) for 5 h prior to staining. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 BV605 RM4-5, CD8 FITC 53-6.7, CD44 APC IM7, CD69 PE-Cy7 H1.2F3, CD134 BV786 OX-86, CD137 PE 1AH2, and CD45R/B220 Per-CP 5.5 RA3-6B2 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained with aTNFα BV650 MP6-XT22 (BD Bioscience).

    Article Title: An mRNA SARS-CoV-2 Vaccine Employing Charge-Altering Releasable Transporters with a TLR-9 Agonist Induces Neutralizing Antibodies and T Cell Memory.
    Article Snippet: GolgiStop (BD Biosciences) was added for the last 10 h of the assay. .. Following stimulation, cells were washed, stained with Aqua live/dead viability dye (Thermo Fisher) in PBS, washed two additional times, and stained with a cocktail of monoclonal antibodies and Fc block: CD16/32, CD4 Ax700 RM4-5, CD8 APC-H7 53-6.7, and CD44 APC IM7 (all BD Bioscience). .. Cells were fixed and permeabilized according to the manufacturer’s protocol (BD Biosciences) and stained for intracellular cytokines with IFNγ PE-Cy7 XMG1.2, TNFα BV650 MP6-XT22, and IL-4 BV786 11B11 (BD Bioscience).

    Incubation:

    Article Title: Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primates
    Article Snippet: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207) to yield RBD-Tetramers. .. Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen, L34957), anti-human IgM (FITC, clone: G20–127, BD cat# 555782, 1:5), anti-human IgG (PE-Cy7, clone: G18–145, BD cat# 561298, 1:20), anti-human CD3 (AF700, clone: SP34-2, BD cat# 557917, 1:20), anti-human PD-1 (BV650, clone: EH12.1, BD cat# 564104, 1:20), anti-human CD20 (PE/Dazzle 594, clone: 2H7, BioLegend cat# 302348, 1:20), anti-human CD4 (APC-Cy7, clone: OKT4, BioLegend cat# 317418, 1:20) and anti-human CXCR5 (PerCP-eF710, clone: MU5UBEE, ThermoFisher cat# 46-9185-42, 1:20). .. Cells were fixed/permeabilized using Transcription Factor Staining Buffer Set (ThermoFisher, cat# 00-5521-00) and further stained with anti-human Bcl-6 (PE, clone: 7D1, BioLegend cat# 358504, 1:20) and anti-human Ki-67 (BV421, clone: 11F6, BioLegend cat# 151208, 1:20).

    Real-time Polymerase Chain Reaction:

    Article Title: Virion proteomics of genetically intact HCMV reveals a regulator of envelope glycoprotein composition that protects against humoral immunity
    Article Snippet: Mouse anti-His (clone His.H8) Thermo Fisher MA1-21315 MACS anti-IgG MicroBeads Miltenyi Biotec 130-048-401 MACS LS Columns Miltenyi Biotec 130-042-401 MACS Separation Buffer Miltenyi Biotec 130-091-221 MACS Pre-Separation Filters (70μM) Miltenyi Biotec 130-095-823 .. Aqua live/dead Thermo Fisher L34966 GolgiStop BD Biosciences 554724 PNGase F NEB P0704 Endo H NEB P0702 Agarose anti-V5 tag Abcam ab1229 Agarose anti-HA tag Abcam ab214758 FastGene 2x IC Green qPCR Universal Mix Geneflow P8-0058 CellBrite® Steady 550 Membrane Staining Kit biotium 30107 Fig. S1. ..

    Membrane:

    Article Title: Virion proteomics of genetically intact HCMV reveals a regulator of envelope glycoprotein composition that protects against humoral immunity
    Article Snippet: Mouse anti-His (clone His.H8) Thermo Fisher MA1-21315 MACS anti-IgG MicroBeads Miltenyi Biotec 130-048-401 MACS LS Columns Miltenyi Biotec 130-042-401 MACS Separation Buffer Miltenyi Biotec 130-091-221 MACS Pre-Separation Filters (70μM) Miltenyi Biotec 130-095-823 .. Aqua live/dead Thermo Fisher L34966 GolgiStop BD Biosciences 554724 PNGase F NEB P0704 Endo H NEB P0702 Agarose anti-V5 tag Abcam ab1229 Agarose anti-HA tag Abcam ab214758 FastGene 2x IC Green qPCR Universal Mix Geneflow P8-0058 CellBrite® Steady 550 Membrane Staining Kit biotium 30107 Fig. S1. ..



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